mouse monoclonal antibody against kgf  (R&D Systems)

Journal: The Journal of endocrinology

Article Title: Fibroblast growth factor 7, secreted by breast fibroblasts, is an interleukin-1beta-induced paracrine growth factor for human breast cells.

doi: 10.1677/joe.0.1770065

Figure Lengend Snippet: Figure 1 Western blot analysis (A) and ELISA (B) of recombinant FGF7 and FGF10 protein using different FGF7 and FGF10 antibodies. Different amounts of recombinant FGF7 or FGF10 protein (0, 0·5, 1, 10, 20 or 100 ng) were resolved on a 12% SDS-PAGE gel and Western blotted with different FGF7 and FGF10 antibodies. The H-73 and C-19 anti-FGF7 were used to detect 1 g/ml in an ELISA in the presence of increasing concentrations (0, 1, 3, 10 or 30 g/ml) of low or high molecular weight heparin or heparin sulphate (+FGF7). The reference controls (FGF7) were performed in the absence of anti-FGF7 antibody. Each point represents the result from triplicate experiments; error bars represent S.E.M.

Article Snippet: The goat polyclonal (AF-251-NA) and mouse monoclonal (MAB251) FGF7 antibodies were acquired from R&D Systems.

Techniques: Western Blot, Enzyme-linked Immunosorbent Assay, Recombinant, SDS Page, High Molecular Weight

Journal: The Journal of endocrinology

Article Title: Fibroblast growth factor 7, secreted by breast fibroblasts, is an interleukin-1beta-induced paracrine growth factor for human breast cells.

doi: 10.1677/joe.0.1770065

Figure Lengend Snippet: Figure 4 Effect of breast fibroblast-CM on epithelial and myoepithelial cell proliferation. The effect of breast fibroblast-CM on cell growth was determined by the MTT assay in (A) purified primary breast epithelial cells which were cultured in different ratios of BCM:CM (1:0, 15;1, 7:1, 3:1, 1:1, 1:3). After 10 days of culture the cells were harvested and growth was estimated. (B) Purified primary breast epithelial cells which were cultured with either BCM or CM from one of the three independent breast fibroblast cultures in the absence or presence of a neutralising FGF7 antibody. (C) The normal breast epithelial cell lines HMT3552 and HBrSV1·6·1 and normal breast myoepithelial cell line HBL100 were incubated with either BCM or CM in the absence (CM) or presence of a neutralising FGF7 antibody (FGF7). Each point represents the meanS.E.M. of n3.

Article Snippet: The goat polyclonal (AF-251-NA) and mouse monoclonal (MAB251) FGF7 antibodies were acquired from R&D Systems.

Techniques: MTT Assay, Cell Culture, Incubation

Journal: The Journal of endocrinology

Article Title: Fibroblast growth factor 7, secreted by breast fibroblasts, is an interleukin-1beta-induced paracrine growth factor for human breast cells.

doi: 10.1677/joe.0.1770065

Figure Lengend Snippet: Figure 5 Effect of FGF7 on the primary epithelial and myoepithelial cell proliferation. (A) Purified primary breast epithelial cells cultured in BCM with 10% FCS or in 0, 1 or 5% FCS after serum starvation were incubated with or without 10 ng/ml FGF7. After 10 days of culture, the cells were harvested and growth was estimated using MTT assays. (B) Purified primary breast myoepithelial cells (day 0) cultured in BCM alone (control) or BCM with FGF1, FGF2 or FGF7 for 10 days were harvested and analysed using MTT assays. Each point represents the meanS.E.M. of n3.

Article Snippet: The goat polyclonal (AF-251-NA) and mouse monoclonal (MAB251) FGF7 antibodies were acquired from R&D Systems.

Techniques: Cell Culture, Incubation, Control

Journal: The Journal of endocrinology

Article Title: Fibroblast growth factor 7, secreted by breast fibroblasts, is an interleukin-1beta-induced paracrine growth factor for human breast cells.

doi: 10.1677/joe.0.1770065

Figure Lengend Snippet: Figure 6 Effect of FGF7 on the proliferation of normal and malignant breast cancer cell lines. Normal and malignant human breast cell lines: HBrSV1·6·1 (A), T47D (B), HMT3552 (C), MDA-MB-231 (D), HBL100 (E), MCF7 (F) and ZR75·1 (G) were cultured in BCM in the presence of 0, 5 or 20 ng/ml FGF7. The murine fibroblast NIH3T3 cells were included as a control (H). The numbers of cells were counted and recorded at day 1, 3, 5, 7 and 10. Each point represents the meanS.E.M. of n3.

Article Snippet: The goat polyclonal (AF-251-NA) and mouse monoclonal (MAB251) FGF7 antibodies were acquired from R&D Systems.

Techniques: Cell Culture, Control

Journal: The Journal of endocrinology

Article Title: Fibroblast growth factor 7, secreted by breast fibroblasts, is an interleukin-1beta-induced paracrine growth factor for human breast cells.

doi: 10.1677/joe.0.1770065

Figure Lengend Snippet: Figure 7 Effect of FGF7 on the morphology and growth of breast organoids. Micrographs of Matrigel-embedded breast organoids before treatment (A) and after treatment with BCM only (B) or BCM with a neutralising anti-FGF1 (C), anti-FGF2 (D) or anti-FGF7 (E), or BCM with FGF1 (F), FGF2 (G) or FGF7(H). (I) Seven independent Matrigel-embedded breast organoid samples were incubated with BCM in the presence or absence of FGF7. After 10 days, the organoids were trypsinised and cell numbers counted. (J) Histogram showing the mean number of cellsS.E.M. in seven samples treated with 20 ng/ml and untreated control wells.

Article Snippet: The goat polyclonal (AF-251-NA) and mouse monoclonal (MAB251) FGF7 antibodies were acquired from R&D Systems.

Techniques: Incubation, Control

Journal: The Journal of endocrinology

Article Title: Fibroblast growth factor 7, secreted by breast fibroblasts, is an interleukin-1beta-induced paracrine growth factor for human breast cells.

doi: 10.1677/joe.0.1770065

Figure Lengend Snippet: Figure 8 Effect of various cytokines and oestrogen receptor ligand and agonists on the release of FGF7 by breast fibroblasts. (A) Purified breast fibroblasts were incubated with different concentrations of IL-1, FGF1, FGF2, FGF8, oestrogen and tamoxifen for 10 days, and the level of FGF7 release into the CM was assayed using ELISA. Each point represents the meanS.E.M. of n3. (B) Purified breast fibroblasts were treated with a range of IL-1 concentrations, 0·001, 0·01, 0·1, 1 and 10 ng/ml, and the amount of FGF7 released assayed using ELISA. Each point represents the meanS.E.M. of n6.

Article Snippet: The goat polyclonal (AF-251-NA) and mouse monoclonal (MAB251) FGF7 antibodies were acquired from R&D Systems.

Techniques: Incubation, Enzyme-linked Immunosorbent Assay